Most self locking nuts are actually designed to be used only once. I, and a million other people, tend to use them more than once. If you have to remove them they should ideally be replaced with new ones. Some settling on a new machine can occur and cause some looseness in fasteners even if they haven't backed off, but if you have to tighten them they may not be working. Even if they are working, adding some LocTite shouldn't do any harm and adds another level of security, a belt and suspenders approach.
Get some blue LocTite and keep it with you. It's normal and recommended to have to check and tighen all the fasteners on an RE periodically, more so during the first 1000 miles or so. This is good practice on any bike and highly recommended on an RE. My general rule is that if I have to tighten it more than once or if it's critical, it gets LocTite.
Scott